10dg gel filtration columns  (Bio-Rad)

Journal: Metallomics : integrated biometal science

Article Title: Evaluation of Cu(i) binding to the E2 domain of the amyloid precursor protein - a lesson in quantification of metal binding to proteins via ligand competition.

doi: 10.1039/c7mt00291b

Figure Lengend Snippet: Fig. 8 Solution spectra of protein fraction I (in solid line) and non-protein fraction II (in dashed line) separated by a desalting gel-filtration column (see inset) from the expts listed in Table 3: (a,b) fraction I (a) from expts 1, 2, 4-7 and fraction II (b) from expts 4- 6; (c,d) fraction I (c) and fraction II (d) from expt 3. Absorbance intensity at λ < 450 nm and λ > 450 nm is shown on the left and right vertical axes, respectively. The spectrum (a) is indistinguishable from the spectrum of each purified protein sample alone.

Article Snippet: Samples of reaction mixtures (B1.0 mL) were applied to a desalting gel filtration column (Econo-Pac 10DG packed with P6-DG gel, Bio-Rad) equilibrated in Mops buffer (pH 7.4, 100 mM NaCl).

Techniques: Filtration, Purification